Microscopy

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PerkinElmer UltraVIEW ERS Spinning Disk Confocal Imager

Confocal microscopy is an optical imaging technique used to increase optical resolution and contrast of a micrograph by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane. It enables the reconstruction of three-dimensional structures from the obtained images.

Our imager includes the PerkinElmer UltraVIEW ERS spinning disk confocal imager with Krypton 561 and Argon 488/515 gas lasers and solid-state lasers emitting at 405, 440, 515, and 640nm. Emission discrimination includes the following filters:

527(W55)
445(W60), 615(W70)
485(W60), 705(W90)
587(W125)

The imaging system utilizes a Zeiss Axiovert 200 inverted microscope equipped with a piezo Z-Focus drive for 3-D imaging. Additionally a Solent brand environmental chamber for live cell imaging is available. Our confocal unit utilizes the following Zeiss objectives:

Images are acquired and analyzed using Volocity software on a Dell PC. Volocity enables the use of automated capture protocols, 3D reconstruction, FRET, colocalization analysis and 3D ratio imaging.

Tutorials:

As with all forms of microscopy sample preparation is key. To be compatible with laser imaging and our objectives working distances samples should be prepared properly and guarded by a 0.17mm (No.1) cover slip against a glass slide.

AMG EVOS fl LED Fluorescent Microscope

Easy to use inverted multichannel LED fluorescence microscope
Screen slides - DAPI, Green and Red fluorescence
Phase contrast transmitted light
Check transfection - easy tools for overlay and counting
Specimen holders are available for slides, multi-well plates, 100mm and 35mm dishes, and various flasks.
LED illumination - no Arc lamp warm up
High resolution monochrome camera
Simple, easy-to-use software
Save images direct to USB drive

Detailed specifications:

Objectives:

4X/0.13 LWD Ph/Fl (works with plastic culture dishes, image through slide rather than coverslip for slides)
10X/0.25 LWD Ph/Fl (works with plastic culture dishes, image through slide rather than coverslip for slides)
20X/0.40 LWD Ph/Fl (works with plastic culture dishes, image through slide rather than coverslip for slides)
40X/0.75 FL corrected for coverglass number 1.5, 0.17 mm

Imaging channels:

Phase contrast transmitted light imaging

Fluorescent LED light cubes with fully variable intensity control:

DAPI (357/44, 447/60)
GFP (470/22, 525/50)
TxRed (585/30, 624/40)

Stage and sample capabilities:

Manual stage with stage adapters for most commonly used sample vessels

Leica DM2000 Upright Fluorescent Microscope

Leica DM2000 Upright Fluorescent Microscope with the following noteworthy specifications:

5x, 10x, 20x, 40x & 100x Brightness synchronizers objective lenses
Blue, Green, Red & BGR filter cubes
Spot Software - Basic

Leica MZ6 Dissecting Fluorescent Microscope